Phytochemical Screening and Hptlc Fingerprinting of Anogeissus Acuminata Extracts

Anogeissus acuminata (family Combretaceae) is a plant widely distributed in western parts of India like North Gujarat, Rajasthan, and Madhya Pradesh. Parts of this plant are used in treatment of different conditions in traditional medicine. The plant is also used in other parts of world such as Thailand for treatment of diabetes mellitus. The plant is evaluated for various pharmacological actions. However, systematic phytochemical data for the plant is lacking. Therefore, we evaluated the methanolic extract of plant qualitatively and quantitatively. The methanolic extract of the leaves and bark of plant were found to possess abundant phenolic compounds namely, tannins and flavonoids. Quantitative determination of these extract revealed a presence of 24.57 and 13.63 %w/w tannin and 14.5 and 57% w/w flavonoids in leaf and bark extract respectively. HPTLC fingerprinting analysis of methanolic extract of leaf and bark with mobile phase (toluene: ethyl acetate: formic acid (4.5:3.0:0.2,v/v/v) confirmed the presence of four peaks in both the extracts with different Rf values at 254nm.